Frequency shift keying demodulators for low-power FPGA applications

Master of ScienceDepartment of Electrical and Computer EngineeringDwight D. DayLow-power systems implemented on Field Programmable Gate Arrays (FPGA) have become more practical with advancements leading to decreases in FPGA power consumption, physical size, and cost. In systems that may need to operate for an extended time independent of a central power source, low-power FPGA’s are now a reasonable option. Combined with research into energy harvesting solutions, a FPGA-based system could operate independently indefinitely and be cost effective. Four simple demodulator designs were implemented on a FPGA to test and compare the performance and power consumption of each. The demodulators were a Counter that tracked the length of the input signal period, a One-Shot that counted the input edges over time, a Phase-Frequency Detector (PFD), and a PFD with preprocessing on the input signal to mitigate distortion introduces by the 1-bit subsampling. The designs demodulated a binary frequency shift keying (BFSK) signal using 10.69MHz and 10.71MHz as the input frequencies and a 1kHz data rate. The signal was 1-bit subsampled at 75kHz to provide the demodulators with a signal containing 15kHz and 35kHz. The design size, power consumption, and error performance of each demodulator were compared. At the frequencies and data rate used, the Counter and One-Shot are the most energy efficient by a significant margin over the PFDs. The error performance was nearly equal for all four. As the BFSK baseband frequencies and especially the data rate are increased, the PFD options are expected to be the better options as the Counter and One-Shot may not react quickly enough

Rapid generation of long synthetic tandem repeats and its application for analysis in human artificial chromosome formation

Human artificial chromosomes (HACs) provide a unique opportunity to study kinetochore formation and to develop a new generation of vectors with potential in gene therapy. An investigation into the structural and the functional relationship in centromeric tandem repeats in HACs requires the ability to manipulate repeat substructure efficiently. We describe here a new method to rapidly amplify human alphoid tandem repeats of a few hundred base pairs into long DNA arrays up to 120 kb. The method includes rolling-circle amplification (RCA) of repeats in vitro and assembly of the RCA products by in vivo recombination in yeast. The synthetic arrays are competent in HAC formation when transformed into human cells. As short multimers can be easily modified before amplification, this new technique can identify repeat monomer regions critical for kinetochore seeding. The method may have more general application in elucidating the role of other tandem repeats in chromosome organization and dynamics

The Role of Human Movement in the Transmission of Vector-Borne Pathogens

Vector-borne diseases constitute a largely neglected and enormous burden on public health in many resource-challenged environments, demanding efficient control strategies that could be developed through improved understanding of pathogen transmission. Human movement—which determines exposure to vectors—is a key behavioral component of vector-borne disease epidemiology that is poorly understood. We develop a conceptual framework to organize past studies by the scale of movement and then examine movements at fine-scale—i.e., people going through their regular, daily routine—that determine exposure to insect vectors for their role in the dynamics of pathogen transmission. We develop a model to quantify risk of vector contact across locations people visit, with emphasis on mosquito-borne dengue virus in the Amazonian city of Iquitos, Peru. An example scenario illustrates how movement generates variation in exposure risk across individuals, how transmission rates within sites can be increased, and that risk within sites is not solely determined by vector density, as is commonly assumed. Our analysis illustrates the importance of human movement for pathogen transmission, yet little is known—especially for populations most at risk to vector-borne diseases (e.g., dengue, leishmaniasis, etc.). We outline several important considerations for designing epidemiological studies to encourage investigation of individual human movement, based on experience studying dengue

Psychometric Properties of the Parent and Teacher Versions of the Strengths and Difficulties Questionnaire for 4- to 12-Year-Olds: A Review

Since its development, the Strengths and Difficulties Questionnaire (SDQ) has been widely used in both research and practice. The SDQ screens for positive and negative psychological attributes. This review aims to provide an overview of the psychometric properties of the SDQ for 4- to 12-year-olds. Results from 48 studies (N = 131,223) on reliability and validity of the parent and teacher SDQ are summarized quantitatively and descriptively. Internal consistency, test–retest reliability, and inter-rater agreement are satisfactory for the parent and teacher versions. At subscale level, the reliability of the teacher version seemed stronger compared to that of the parent version. Concerning validity, 15 out of 18 studies confirmed the five-factor structure. Correlations with other measures of psychopathology as well as the screening ability of the SDQ are sufficient. This review shows that the psychometric properties of the SDQ are strong, particularly for the teacher version. For practice, this implies that the use of the SDQ as a screening instrument should be continued. Longitudinal research studies should investigate predictive validity. For both practice and research, we emphasize the use of a multi-informant approach